Search results for "Giardia duodenalis"

showing 3 items of 3 documents

Occurrence and genotypes of Giardia isolated from lambs in Spain

2009

Three hundred and eighty six faecal specimens were randomly collected from 1- to 3-month-old lambs from 16 farms in Spain to investigate the presence of different genotypes of Giardia duodenalis. Individual specimens were examined by IFA (Immunofluorescence assay) and beta-giardin PCR polymerase chain reaction. Cysts of G. duodenalis were shed by lambs in every flock analyzed, showing a prevalence by farms of 100%. The average prevalence of G. duodenalis for the 386 specimens was 42%, ranging from 8.3 to 80% depending on the farm. beta-giardin PCR positive samples were sequenced to determine the genotypes present at each farm and seven new subtypes of beta-giardin Assemblage E are reported …

GiardiasisVeterinary medicineGenotypeanimal diseasesMolecular Sequence DataHigh variabilityProtozoan ProteinsFluorescent Antibody TechniqueSheep DiseasesBiologyPolymerase Chain ReactionGenetic analysislaw.inventionMicrobiologyFeceslawZoonosesGenotypePrevalenceAnimalsPolymerase chain reactionSheepGiardiaGiardiaSequence Analysis DNAbiology.organism_classificationCytoskeletal ProteinsInfectious DiseasesSpainGiardia duodenalisParasitologyFlockParasitology International
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One Health Approach to Zoonotic Parasites: Molecular Detection of Intestinal Protozoans in an Urban Population of Norway Rats, Rattus norvegicus, in …

2021

Rattus norvegicus, the brown or Norway rat, is the most abundant mammal after humans in urban areas, where they live in close proximity to people. Among rodent-borne diseases, the reservoir role of Norway rats of zoonotic parasites in cities has practically been ignored. Considering the parasitic diseases in the One Health approach, we intended to identify and quantify the zoonotic intestinal protozoans (ZIP) in an urban population of R. norvegicus in the city of Barcelona, Spain. We studied the presence of ZIP in 100 rats trapped in parks (n = 15) as well as in the city’s sewage system (n = 85) in the winter of 2016/17. The protozoans were molecularly identified by means of a multiplex PCR…

Microbiology (medical)BarcelonaParàsits<i>Giardia duodenalis</i>PopulationDientamoeba fragilislcsh:MedicineSewageZoologyBiology<i>Cryptosporidium</i> spp.Cryptosporidium spp.Article<i>Blastocystis</i>Cryptosporidium sppOne Health approachZoonosesUrbanizationMicroorganismes patògensPandemicparasitic diseasesImmunology and AllergyeducationMolecular BiologyDientamoeba fragilis<i>Dientamoeba fragilis</i><i>Rattus norvegicus</i>education.field_of_studyBlastocystisGeneral Immunology and Microbiologybusiness.industrylcsh:RCryptosporidiumbiology.organism_classificationRattus norvegicuszoonosesInfectious DiseasesOne HealthBlastocystisbusinessGiardia duodenalis
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Molecular characterization of Giardia duodenalis cysts in the Oreto river (Sicily, Southern Italy)

2012

&lt;p&gt;&lt;strong&gt;Background&lt;/strong&gt;: The presence of Giardia was investigated along the Oreto river between January 2008 and May 2009 with the aim of understanding the source of contamination by molecular typing of cysts.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods&lt;/strong&gt;: A total of 38 water samples (10 collected from the river mouth, 24 from the whole Oreto basin and 4 sewage samples from the Monreale treatment plant) were processed. In addition, 22 faecal samples of livestock living close to the Oreto area, were analyzed. The presence of Giardia was determined by immunofluorescence assay and their genetic characterization was achieved by a nested PCR assay targeting the …

contaminationGiardia duodenalis oreto river Giardia cystsGiardialcsh:Public aspects of medicinelcsh:RGiardia;Oreto River; contaminationDOAJ:Public Healthlcsh:MedicineOreto Riverlcsh:RA1-1270Settore MED/42 - Igiene Generale E ApplicataDOAJ:Health Sciences
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